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2 edition of effect of aluminum detoxification on phospholipid biosynthesis in pseudomonas fluorescens found in the catalog.

effect of aluminum detoxification on phospholipid biosynthesis in pseudomonas fluorescens

Angie Kirwan

effect of aluminum detoxification on phospholipid biosynthesis in pseudomonas fluorescens

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Published by Laurentian University in Sudbury, Ont .
Written in English


Edition Notes

StatementAngie Kirwan.
The Physical Object
Pagination48 l. :
Number of Pages48
ID Numbers
Open LibraryOL22161418M

Mitochondria move, fuse and divide in cells. The dynamic behavior of mitochondria is central to the control of their structure and function. Three conserved mitochondrial dynamin-related GTPases (i.e., mitofusin, Opa1 and Drp1 in mammals and Fzo1, Mgm1 and Dnm1 in yeast) mediate mitochondrial fusion and by: Full text of "Plant Growth & Health Promoting Bacteria" See other formats.   The opportunistic pathogen Pseudomonas aeruginosa (PA) produces and secretes the biosurfactant rhamnolipid (RL) which modulates bacterial biofilm. Here, we show that monomeric RL enhances the ability of αSN to permeabilize membranes, while micellar RL rapidly induces protein β‐sheet structure with a worm‐like fibrillary appearance, which Cited by: 2.


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effect of aluminum detoxification on phospholipid biosynthesis in pseudomonas fluorescens by Angie Kirwan Download PDF EPUB FB2

Phospholipid Biosynthesis and Solvent Tolerance in Pseudomonas putida Strains [Holly C Finkart] on *FREE* shipping on qualifying offers. 13CNMR and 1HNMR studies revealed that aluminum citrate (Al-citrate) was metabolized intracellularly and that oxalic acid was an important product in the Al-stressed cells.

This dicarboxylic acid was produced via the oxidation of glyoxylate, a precursor generated through the cleavage of isocitrate. In the control cells, citrate was biotransformed essentially with the Cited by: 9. The soil microbe Pseudomonas fluorescens has been shown to detoxify aluminum by the elaboration of a soluble metabolite where the trivalent metal is sequestered [Appanna andFEMS Microbiol.

Lett. 24 () –]. The inclusion of 5 mM iron in the growth medium elicited an entirely disparate detoxification by: Pseudomonas aeruginosa, an opportunistic human pathogen is known to synthesize rhamnolipid and polyhydroxyalkanoic acid (PHA) of which the acyl-group precursors (e.g., (R)hydroxydecanoic acid) are provided through RhlA and PhaG enzyme, respectively, which have 57% gene sequence homology.

The inhibitory effect of three 2-bromo-fatty acids of 2 Cited by: 1. dokl akad nauk sssr. jul 1; [effect of the sources of carbon on the biosynthesis of alpha-glutaric acid in cultures of pseudomonas fluorescens].Author: Shapashnikov Vn, Kosheleva Na, Kolesnikova Ig, Baikova La.

Phospholipids are major constituents of the membranes that separate cells from their environment and compartmentalize eukaryotic cells into functional units. In yeast (Saccharomyces cerevisiae), the expression of genes involved in phospholipid biosynthesis is regulated by a mechanism that respondsCited by: 2.

The seeds were either supplied (h) or not (s) with the biological control agent Pseudomonas fluorescens DR54 at 5 10 8 cells. The day after inoculation is shown in the individual markers.

This chapter discusses the biosynthesis of phospholipids, which consists of (1) the basic pathways leading to de novo formation of phospholipid and (2) special transformations that interconvert one phospholipid to another. There is more than one way that nature has developed to make specific phospholipids, and often, different species or subcellular organelles have Cited by:   PHOSPHOLIPID METABOLISM & GLYCOLIPID METABOLISM DComC.

Phospholipids steve. Economic and industrial importance of fungi Natheer. Economic importance of fungi Meentu Prakash.

Chemistry of Phospholipids rohini sane. Mitochondria Donna Penton. Lipoproteins Alaa El Badrawy. phospholipid biosynthesis in yeast). A novel DG pyrophosphate phosphatase has also been identified in yeast [4]. The enzyme has PA phosphatase activity but prefers DG pyrophosphate as substrate.

This phosphatase has broad specificity but only DG pyrophosphate and PA have been shown to be substrates in vivo. Abstract. Pseudomonas aeruginosa produces exoproducts correlated with its pathogenicity.

One of these virulence-associated traits is the surfactant rhamnolipid. The production of alginate and lipopolysaccharide (LPS) are also of importance for P. aeruginosa virulence. The product of the algC gene (which is involved in alginate production through its Cited by: RESEARCH PAPER.

Growth kinetics, effect of carbon substrate in biosynthesis of mcl-PHA by Pseudomonas putida Bet A.M. Gumel I; M.S.M. Annuar I; T.

Heidelberg II. I Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia II Department of Chemistry, Faculty of Science, University of Malaya, Kuala Effect of aluminum detoxification on phospholipid biosynthesis in pseudomonas fluorescens book, Malaysia.

Growth kinetics, effect of carbon substrate in biosynthesis of mcl-PHA by Pseudomonas putida Bet A.M. Gumel1, M.S.M. Annuar1, T. Heidelberg2 1Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia. 2Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.

Pseudomonas aeruginosa, an opportunistic human pathogen is known to synthesize rhamnolipid and polyhydroxyalkanoic acid (PHA) of which the acyl-group precursors (e.g., (R)hydroxydecanoic acid.

Abstract. The biological control agent (BCA) Pseudomonas fluorescens DR54 was applied to seeds (experiment 1) or roots (experiment 2) of barley growing in microcosms, while noninoculated plants served as controls. The fate of the BCA and its effects on the rhizosphere microbial community was evaluated in microcosms destructively sampled at days 2, 4, 6, and Cited by: Chem* Lecture Phospholipid Biosynthesis Fats and phospholipids share the same initial synthesis pathway Fats and phospholipids both contain fatty acids linked by ester bonds to a glycerol backbone, and are described as glycerolipids.

The synthesis pathway starts by reducing dihydroxyacetone phosphateFile Size: 48KB. E-Mail Address. Password. Forgotten Password. Remember Me. «hide 10 20 30 40 50 mgifdyknlg tegsktlfad amaitlysyh nldngfavgy qhnglglglp 60 70 80 90 atlvgallgs tdsqgvipgi pwnpdsekaa leavqkagwt pisasalgya gkvdargtff gekagyttaq vevlgkydda gklleigigf rgtsgpretl isdsigdlis dllaalgpkd yaknyageaf ggllknvady agahgltgkd vvvsghslgg lavnsmadls nykwagfykd anyvayaspt.

Phospholipid Biosynthesis and Solvent Tolerance in Pseudomonas putida Strains HOLLY C. PINKART1,2* AND DAVID C. WHITE1,2,3 Center for Environmental Biotechnology, The University of Tennessee, Knoxville, Tennessee ,1 Department of Microbiology, The University of Tennessee, Knoxville, Tennessee ,2.

The annotations for P. aeruginosa PAO1 (PseudoCyc) pathways were computationally derived and subjected to manual curation by the Bioinformatics Research Group at SRI and the Pseudomonas Genome Database. News. April 3, Added missing enzymes to nitrate reduction I (denitrification).

Ma New and updated pathways. Pyoverdine 1. Pseudomonas fluorescens Pf is one of the model organisms for biofilm research.

Our previous transposon mutagenesis study suggested a requirement for the de novo purine nucleotide biosynthesis pathway for biofilm formation by this organism.

This study was performed to verify that observation and investigate the basis for the defects in biofilm. For the genus Pseudomonas, many structurally different CLPs have been identified.

CLPs play an important role in surface motility of Pseudomonas strains, but also in virulence and attachment/detachment to and from surfaces.

In this Ph.D. thesis project, two new CLP biosynthesis clusters were identified in Pseudomonas fluorescens and fully Cited by: 1. Abstract. In the yeast Saccharomyces cerevisiae, the major membrane phospholipid phosphatidylcholine is synthesized by the CDP-diacylglycerol and CDP-choline examined the regulation of phosphatidylcholine synthesis by CTP.

The cellular concentration of CTP was elevated (fold) by overexpressing CTP synthetase, the enzyme. ADVERTISEMENTS: In this article we will discuss about: 1. Distribution of Phospholipids 2. Classification of Phospholipids 3.

Synthesis 4. Functions 5. Role of Endocrines. Distribution of Phospholipids: The salient facts about the distribution of phospholipids are summarized below: i. The phospholipids are widely distributed in the body and are contained within it. They remain. the production of extracellular lipase by Pseudomonas fluorescens KE38 Pseudomonas fluorescens KE38 was grown at 25 °C with shaking ( rpm) for 24 h in 10 mL of minimal medium (50 mM Na 2 HPO H 2 O, 20 mM KH 2 PO 4, 10 mM NaCl, 20 mM NH 4 Cl, 2 mM MgSO 4, and mM CaCl 2) supplemented with 1% (v/v) olive oil as carbon and energy source.

Abstract. Me 2 SO is a polar solvent that is widely used in biochemistry, pharmacology, and industry. Although there are several reports in the literature concerning the biological effects of Me 2 SO, the total cellular response remains unclear.

In this paper, DNA microarray technology combined with the hierarchical clustering bioinformatics tool was used. Sphingolipids are essential components of the plasma membrane in all eukaryotic cells.

cerevisiae cells make three complex sphingolipids: inositol-phosphoceramide (IPC), mannose-inositol-phosphoceramide (MIPC), and mannose.

OXIDATION-REDUCTION POTENTIAL AND GROWTH OF SALMONELLA SPP. AND PSEUDOMONAS FLUORESCENS. Iowa State University, Ph.D., Food Technology, University Microfilms, A XEROX Company, Ann Arbor, Michigan THIS DISSERTATION HAS BEEN MICROFILMED EXACTLY AS RECEIVED. Both Pseudomonas aeruginosa and Pseudomonas fluorescens secrete a lipase into the extracellular medium.

Unlike the lipase of P. aeruginosa, the lipase produced by P. fluorescens does not contain any N‐terminal signal show that the P. fluorescens lipase is secreted through the signal peptide‐independent pathway of the alkaline protease that we.

Biosynthesis of phospholipids. Biosynthesis of phospholipids. Every tissue synthesize phospholipids, but at dissimilar rates. In all tissues apart from the liver, phospholipids are synthesized, used and degraded in situ; when in liver large proportion of the phospholipids later than the synthesis is transferred to the plasma and like a matter of fact, liver is practically the /5(K).

Phospholipid Biosynthesis Phospholipid Biosynthesis Moore, T S Phospholipids have long been recognized as major components of biological membranes. As such, these molecules have a profound influence on cellular activities through control of membrane permeability and membrane-bound enzyme activities.

Interestingly, very high similarity values (79–%) were detected to predicted proteins of various Pseudomonas species such as strains of P. aeruginosa, P. putida, Pseudomonas fluorescens, Pseudomonas syringae, Pseudomonas entomophila, Pseudomonas stutzeri and Pseudomonas by: Aromatic amino acid biosynthesis in S.

cerevisiae is controlled by a combination of feedback inhibition, activation of enzyme activity, and regulation of enzyme synthesis (CITS:[Jones][]).

The carbon flow through the pathways is regulated primarily at the initial step and the branching points by the terminal end-products. ABSTRACT This study focused on two separate topics.

First, potentially beneficial products from the bacterium Pseudomonas fluorescens were investigated and in the second, methods were developed for the detection of toxins produced by the blue-green alga Microcystis aeruginosa.

Isolation and host range characterization of promising lytic phages. The aim of this work was to isolate, select and characterize an effective lytic phage for a range of P. fluorescens strains. Therefore, initially several phages were isolated from raw sewage of a wastewater treatment plant that receives effluents from different dairy by: What type of fat can make the phospholipid bilayer more fluid.

less fluid. Unsaturated fats/saturated fats What makes up as much as 50% of membrane lipids, fills gaps left by unsaturated fatty acids, which has the effect of decreasing membrane fluidity but also increases the strength of the membrane. Lipids metabolism in plants 1.

Lipids metabolism Somayeh Hooshyar 2. Definition of lipids • Lipids are a structurally diverse group of hydrophobic molecules that are preferentially soluble in non-aqueous solvents such as chloroform • Lipids are highly hydrophobic molecules with major roles in membrane structure, storage of carbon and energy reserves, plant.

assess the antimicrobial effect of phospholipid compound produced and activity was tested against test organisms (E. coli, Pseudomonas aeruginosa, Candida tropicalis, and Staphylococcus aureus). Methodology Collection of water sample: Soil sample was collected from Lonar l Temperature and pH of Lonar lake was recorded.

2A Effect of incubation period on PLFA concentration and composition of bacteria Bacterial cultures B. licheniformis, B. subtilis and A. hydrophila were grown in BSS-GYP medium as described above.

Each culture was grown in 1L x 3 flasks containing ml of the BSS-GYP medium over a period of h. At specific time. Evaluation of PHA synthesis on different Pseudomonas putidastrains in shake flask experiments. We next challenged Pseudomonas putida KT, KT, S12, and F1 to grow in minimal medium (M9) supplemented with 30 (g/L) of crude glycerol as the sole carbon source in batch cultures.

After 72 hours of cultivation, cells were harvested so as to quantify Cited by:. In Pseudomonas fluorescens, when phosphate levels are high, the activity of the enzyme _____ is also high and c-di-GMP is formed.

Conversely, when phosphate levels are low, _____ activity is low and c-di-GMP is destroyed.Isolation and Characterization of a Pseudomonas aeruginosa Strain DN1 Degrading p-Nitrophenol.

Research Journal of Microbiology, 3: DOI: /jmPseudomonas Durrett and Levin,; Kerr etal., ). Similarly, multiple strains of Pseudomonas fluorescens can evolve and coexist in spa-tially heterogeneous microcosms (Rainey and Travisano, ), and siderophore production by P.

aeruginosa is less susceptible to cheating when dispersal is limited (Kümmerli etal., a). Christensen etal.